Journal: Frontiers in Microbiology

Article Title: Identification and Characterization of New Bacteriophages to Control Multidrug-Resistant Pseudomonas aeruginosa Biofilm on Endotracheal Tubes

doi: 10.3389/fmicb.2020.580779

Figure Lengend Snippet: Lytic spectrum and efficiency of plating of the bacteriophages against sensible and multidrug-resistant Pseudomonas aeruginosa isolated from different sources.

Article Snippet: Host range was evaluated by the presence of clear or turbid inhibition haloes in a spot test on double-layer agar against 26 clinical isolates and seven multidrug-resistant P. aeruginosa strains (Panel ATCC ® MP-23 TM ; ).

Techniques: Isolation

Journal: Frontiers in Microbiology

Article Title: Identification and Characterization of New Bacteriophages to Control Multidrug-Resistant Pseudomonas aeruginosa Biofilm on Endotracheal Tubes

doi: 10.3389/fmicb.2020.580779

Figure Lengend Snippet: Genomic properties of Pseudomonas aeruginosa bacteriophages.

Article Snippet: Host range was evaluated by the presence of clear or turbid inhibition haloes in a spot test on double-layer agar against 26 clinical isolates and seven multidrug-resistant P. aeruginosa strains (Panel ATCC ® MP-23 TM ; ).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Identification and Characterization of New Bacteriophages to Control Multidrug-Resistant Pseudomonas aeruginosa Biofilm on Endotracheal Tubes

doi: 10.3389/fmicb.2020.580779

Figure Lengend Snippet: (A) Optical density of planktonic Pseudomonas aeruginosa strains after 2, 4, 6, 8, and 24 h of co-incubation with different bacteriophages. (B) Anti-biofilm activity of different bacteriophages on biofilm viability rates expressed in log 10 CFU/cm 2 (left y axis) and metabolic activity expressed in absorbance at 492 nm (right y axis). Inhibition of planktonic growth and anti-biofilm activity assays was carried out at three independent times in triplicate against 15 strains of P. aeruginosa . (C–H) Scanning electron micrographs after phage treatment: (C) control, (D) vB_PaeM_USP_1, (E) vB_PaeM_USP_2, (F) vB_PaeM_USP_3, (G) vB_PaeM_USP_18, and (H) vB_PaeM_USP_25. Scale bar = 100 nm. After the treatment of bacteriophages, it is possible to observe lower planktonic growth, biofilm viability rates, and metabolic activity. Disrupted biofilms and cell debris are also noticed in SEM images.

Article Snippet: Host range was evaluated by the presence of clear or turbid inhibition haloes in a spot test on double-layer agar against 26 clinical isolates and seven multidrug-resistant P. aeruginosa strains (Panel ATCC ® MP-23 TM ; ).

Techniques: Incubation, Activity Assay, Inhibition, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Probiotics as a New Regulator for Bone Health: A Systematic Review and Meta-Analysis

doi: 10.1155/2021/3582989

Figure Lengend Snippet: Characteristics of studies that reported the relationship between probiotic consumption and bone health in animals.

Article Snippet: Schepper et al. (2019) , Eleven-week-old male BALB/c mice , 88 ( n = 10–18 per group) , 1. ABX: antibiotic-treated (ampicillin 1.0 g/L and neomycin 0.5 g/L) 2. ABX + LR 3. ABX + LGG 4. ABX + EC 5. ABX + MDY (high-molecular-weight polymer) , 300 mL of L. reuteri 6475 (LR), Lactobacillus rhamnosus (LGG), nonpathogenic Escherichia coli (EC; ATCC O6 : B1) (1 × 10 9 CFU/mL) , Ctrl: normal diet , 4 week , BMD (mg/cc) , Ctrl: 1019 ± 24.34 ABX: 1024 ± 18.5 ABX + LR: 1045 ± 39.05 ABX + LGG: 1044 ± 55.7 ABX + EC: 1043 ± 27.23 ABX + MDY: 1021 ± 27.02.

Techniques: Control, Milk, Bacteria, Synthesized, Calcium Carbonate, Saline, Modification, Starch, Concentration Assay, Knock-Out, Negative Control, Positive Control, Polymer, Mouse Assay